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fusion snap assembly master mix  (TaKaRa)


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    Structured Review

    TaKaRa fusion snap assembly master mix
    Fusion Snap Assembly Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/in+fusion+snap+assembly+system/In-Fusion+Snap+Assembly+Master+Mix/pmc13049534-67-21-28
    Average 98 stars, based on 1070 article reviews
    fusion snap assembly master mix - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Expressing:

    Article Title: A SARS-CoV-2 entry inhibitor trimerizes to lock the spike protein in a closed conformation
    Article Snippet: The immunoadhesin forms of coronavirus receptor-binding domains (RBD-Fcs) and soluble human ACE2 were produced as described previously . .. The SARS2 nanobody N3113 expression plasmid was generated by ligating synthesized gBlocks (IDT) in frame with the human IgG1 Fc domain into the pCAGGS vector using the In-fusion Snap Assembly system (Takara, 638948). .. The resulting constructs were transiently transfected into Expi293F cells using PEI MAX (Kyfora Bio, 24765).

    Plasmid Preparation:

    Article Title: A SARS-CoV-2 entry inhibitor trimerizes to lock the spike protein in a closed conformation
    Article Snippet: The immunoadhesin forms of coronavirus receptor-binding domains (RBD-Fcs) and soluble human ACE2 were produced as described previously . .. The SARS2 nanobody N3113 expression plasmid was generated by ligating synthesized gBlocks (IDT) in frame with the human IgG1 Fc domain into the pCAGGS vector using the In-fusion Snap Assembly system (Takara, 638948). .. The resulting constructs were transiently transfected into Expi293F cells using PEI MAX (Kyfora Bio, 24765).

    Article Title: Rapid activation of ARF6 after RAF inhibition augments BRAF V600E and promotes therapy resistance.
    Article Snippet: .. ARF6WT-V5 and ARF6Q67L-V5 were inserted into the p-TRPIZ vector using the In-Fusion Snap Assembly system (Takarabio, Cat# 638945). .. HEK-293T cells were cotransfected with 2nd generation lentivirus packaging vectors (5 μg pCMVGag/Pol, Addgene, Cat#35614; 1 μg pCMV-VSVG, Addgene, Cat# 8454) and 5 μg of expression constructs (including piSMART-hEF1a-TurboGFP-shARF6, see Key Resource Table) using LipofectamineTM 3000 Transfection Reagent (Thermo Scientific, Cat# L3000008).

    Generated:

    Article Title: A SARS-CoV-2 entry inhibitor trimerizes to lock the spike protein in a closed conformation
    Article Snippet: The immunoadhesin forms of coronavirus receptor-binding domains (RBD-Fcs) and soluble human ACE2 were produced as described previously . .. The SARS2 nanobody N3113 expression plasmid was generated by ligating synthesized gBlocks (IDT) in frame with the human IgG1 Fc domain into the pCAGGS vector using the In-fusion Snap Assembly system (Takara, 638948). .. The resulting constructs were transiently transfected into Expi293F cells using PEI MAX (Kyfora Bio, 24765).

    Article Title: Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain.
    Article Snippet: Electrophoresis was run on ice, starting at 150 V for 2 h and continued at 250 V for 2 h. The gels were stained with Coomassie blue stain (catalog no. 50-109-1216, Anatrace). .. Nb2 mutants were generated via site-directed mutagenesis using a Takara In-Fusion Snap Assembly system (catalog no. 638951, Takara Bio, Shiga, Japan) and purified as described above. .. For mROS, 6-8 retinas from6-8-week-oldwild-type C57B6/Jmice (Jackson Laboratory, ME, USA) were isolated and placed in ice-cold Ringer’s buffer (10mM HEPES, 130mM NaCl, 3.6mM KCl, 12mM MgCl2, 0.02mM EDTA, pH 7.4).

    Article Title: Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain
    Article Snippet: Electrophoresis was run on ice, starting at 150 V for 2 h and continued at 250 V for 2 h. The gels were stained with Coomassie blue stain (catalog no. 50-109-1216, Anatrace). .. Nb2 mutants were generated via site-directed mutagenesis using a Takara In-Fusion Snap Assembly system (catalog no. 638951, Takara Bio, Shiga, Japan) and purified as described above. .. For mROS, 6-8 retinas from 6-8-week-old wild-type C57B6/J mice (Jackson Laboratory, ME, USA) were isolated and placed in ice-cold Ringer’s buffer (10 mM HEPES, 130 mM NaCl, 3.6 mM KCl, 12 mM MgCl 2 , 0.02 mM EDTA, pH 7.4).

    Synthesized:

    Article Title: A SARS-CoV-2 entry inhibitor trimerizes to lock the spike protein in a closed conformation
    Article Snippet: The immunoadhesin forms of coronavirus receptor-binding domains (RBD-Fcs) and soluble human ACE2 were produced as described previously . .. The SARS2 nanobody N3113 expression plasmid was generated by ligating synthesized gBlocks (IDT) in frame with the human IgG1 Fc domain into the pCAGGS vector using the In-fusion Snap Assembly system (Takara, 638948). .. The resulting constructs were transiently transfected into Expi293F cells using PEI MAX (Kyfora Bio, 24765).

    Mutagenesis:

    Article Title: Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain.
    Article Snippet: Electrophoresis was run on ice, starting at 150 V for 2 h and continued at 250 V for 2 h. The gels were stained with Coomassie blue stain (catalog no. 50-109-1216, Anatrace). .. Nb2 mutants were generated via site-directed mutagenesis using a Takara In-Fusion Snap Assembly system (catalog no. 638951, Takara Bio, Shiga, Japan) and purified as described above. .. For mROS, 6-8 retinas from6-8-week-oldwild-type C57B6/Jmice (Jackson Laboratory, ME, USA) were isolated and placed in ice-cold Ringer’s buffer (10mM HEPES, 130mM NaCl, 3.6mM KCl, 12mM MgCl2, 0.02mM EDTA, pH 7.4).

    Article Title: Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain
    Article Snippet: Electrophoresis was run on ice, starting at 150 V for 2 h and continued at 250 V for 2 h. The gels were stained with Coomassie blue stain (catalog no. 50-109-1216, Anatrace). .. Nb2 mutants were generated via site-directed mutagenesis using a Takara In-Fusion Snap Assembly system (catalog no. 638951, Takara Bio, Shiga, Japan) and purified as described above. .. For mROS, 6-8 retinas from 6-8-week-old wild-type C57B6/J mice (Jackson Laboratory, ME, USA) were isolated and placed in ice-cold Ringer’s buffer (10 mM HEPES, 130 mM NaCl, 3.6 mM KCl, 12 mM MgCl 2 , 0.02 mM EDTA, pH 7.4).

    Purification:

    Article Title: Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain.
    Article Snippet: Electrophoresis was run on ice, starting at 150 V for 2 h and continued at 250 V for 2 h. The gels were stained with Coomassie blue stain (catalog no. 50-109-1216, Anatrace). .. Nb2 mutants were generated via site-directed mutagenesis using a Takara In-Fusion Snap Assembly system (catalog no. 638951, Takara Bio, Shiga, Japan) and purified as described above. .. For mROS, 6-8 retinas from6-8-week-oldwild-type C57B6/Jmice (Jackson Laboratory, ME, USA) were isolated and placed in ice-cold Ringer’s buffer (10mM HEPES, 130mM NaCl, 3.6mM KCl, 12mM MgCl2, 0.02mM EDTA, pH 7.4).

    Article Title: Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain
    Article Snippet: Electrophoresis was run on ice, starting at 150 V for 2 h and continued at 250 V for 2 h. The gels were stained with Coomassie blue stain (catalog no. 50-109-1216, Anatrace). .. Nb2 mutants were generated via site-directed mutagenesis using a Takara In-Fusion Snap Assembly system (catalog no. 638951, Takara Bio, Shiga, Japan) and purified as described above. .. For mROS, 6-8 retinas from 6-8-week-old wild-type C57B6/J mice (Jackson Laboratory, ME, USA) were isolated and placed in ice-cold Ringer’s buffer (10 mM HEPES, 130 mM NaCl, 3.6 mM KCl, 12 mM MgCl 2 , 0.02 mM EDTA, pH 7.4).



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